Vacuolar inheritance. Mother cells donate approximately 50% of their vacuolar material to growing buds. Protrusions from the vacuole grow toward the emerging bud, and a scission event leads to the formation of vesicles, which travel into the bud. A homotypic fusion event between these vesicles (consisting of a priming event, docking, and, finally, fusion) forms the vacuole of the daughter cell.