Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 9;62(2):894–904. doi: 10.1093/rheumatology/keac272

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of the British Society for Rheumatology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Fig. 2 — Decreased overall FN1 expression and change of FN1 transcript ratios results in decreased matrix deposition

(A) Representative images of Alcian blue staining of neo-cartilage pellets of primary chondrocytes transduced with non-targeting shRNA (control) and FN1 targeting shRNA (FN1). (B) Quantification of Alcian blue (AB) pixel intensity staining of control and FN1 targeting shRNA transduced pellets (n = 9). Colours of dots represent the different donors. (C) sGAG content normalized to DNA content in pellets of the control (n = 7) and FN1 group (n = 11) analysed by dimethylmethylene blue assay. P-values were determined by GEEs, with experimental readout as the dependent variable and donor and group as covariates. *P < 0.05, ***P < 0.005. Colour version is available at Rheumatology online.