Fig. 1.

ELF3 expression is induced in synovial fibroblasts and synovial tissue by co-stimulation with IL-17A and TNF

Human primary synovial fibroblasts from RA and OA patients or synovial tissue explants of RA patients were stimulated with IL-17A (50 ng/ml) and/or TNF (10 ng/ml) or left untreated. (A) ELF3 mRNA expression in RA and OA synovial fibroblasts stimulated for 24 h. (B) ELF3 mRNA expression in RA synovial tissue explants stimulated for 16 h. (C) ELF3 immunofluorescence in OA synovial fibroblasts stimulated for 24 h. (D) Time course of ELF3 mRNA expression in OA synovial fibroblasts. Data represent means ± SEM of three (A, D) or four (B) individual experiments carried out with cells and synovial explants from different donors. *P < 0.05, **P < 0.01, and ***P < 0.001 for indicated comparisons. #P < 0.05 compared with control. In time series experiment groups were compared separately at each time point. DAPI: 4′,6-diamidino-2-phenylindole; ELF3: E74-like factor-3.