Fig. 5.

ELF3 silencing reduces the induction of inflammatory factors by the combination of IL-17A and TNF

Human primary synovial fibroblasts from OA patients were transfected with ELF3 siRNA or with negative control siRNA. After 48 h the cells were stimulated for 24 h with IL-17A (50 ng/ml) and/or TNF (10 ng/ml). (A) mRNA expression of the indicated cytokines and MMPs analysed by qRT-PCR. (B) Protein concentration of the indicated cytokines and MMPs in the cell supernatant analysed by Bio-Plex multiplex immunoassay. Data represent means ± SEM of six individual experiments with cells from different donors. *P <  0.05, **P < 0.01, ***P < 0.001. CCL8: chemokine (C-C motif) ligand 8; CSF3: colony-stimulating factor-3; CXCL5: chemokine (C-X-C motif) ligand-5; ELF3: E74-like factor-3.