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. 2023 Jan 19;12:e82217. doi: 10.7554/eLife.82217

Figure 2. iTfh17 cells are superior in memory maintenance.

Figure 2.

(A–H) 5×104 FACS-purified OT-II iTfh1, iTfh17, or iTfh2 cells were separately transferred to CD28KO recipients. The early immunization group was immunized by OVA or NP-OVA in alum one day after the adoptive cell transfer. The late immunization group was immunized by the same antigens 35 days after the adoptive cell transfer. Spleens or serum were collected on day 7 after the immunization. Experiment design (A), representative FACS plots (B, C, D) and statistics (E, F, G) showing the percentages of Tfh cells in OT-II cells, the percentages of BGC in total B cells and the percentages of BASC in total B cells. For antibody titers, statistic (H) showing OD405 values of anti-NP2 and anti-NP23 total IgG. (I–J) Tfh1/2/17 cells from mouse splenocytes were analyzed for CCR7 and CD44 expression. Representative FACS plots (I). and statistics (J) showing the expressions of CCR7 and CD44. The p values were calculated by one-way ANOVA. The results in (E, F, G, H, I, J) were both pooled from two independent experiments. Source data for the statistics can be found in Figure 2—source data 1.

Figure 2—source data 1. Source data file of statistics in Figure 2.