Fig. 4.

Regnase-1 self-regulatory mechanisms and therapeutic strategy manipulating such pathway. A Regnase-1 harbors two distinct stem-loop structures at its 3’ UTR, which are responsible for Regnase-1-dependent degradation of self mRNAs. Such negative-feedback pathway may be a backup mechanism to regulate Regnase-1 abundance. B Targeting of two morpholino oligonucleotides (MO) to the right-arm of each stem-loop structures in Regnase-1 3’ UTR interferes the stem-loop conformation, which blocks Regnase-1 association to its own mRNA and thereby increases its availability. The increased Regnase-1 protein abundance enables heightened degradation of factors related to inflammation. This figure is made with BioRender: https://biorender.com/