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. 2022 Nov 28;14(1):243–259. doi: 10.1002/jcsm.13124

Figure 7.

Figure 7

Oxygen consumption and ATP production in isolated mitochondria is decreased in aged mice, but partially rescued in Aged TKO. Maximal respiration of isolated mitochondria from mixed quad/gastroc with glutamate/malate/succinate (Glu/M/S) substrates, followed by addition of rotenone (Rot), and oligomycin (Oligo) (A). Maximal ATP production of isolated mitochondria with Glu/M/S substrates (B) (n = 5–13 per group). Images (C) and quantification (D) of total muscle fibres from succinate dehydrogenase (SDH) stained plantaris muscles (n = 4–8 per group, scale bar: 500 μm). Images (E) and quantification (F) of mitochondrial area on TEM images from plantaris muscle (n = 6–10 per group, scale bar 3 μm). *P < 0.05, **P < 0.01 as indicated by 2‐way ANOVA.