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. 2022 Dec 5;614(7946):136–143. doi: 10.1038/s41586-022-05605-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — (a-b) Synchronization scheme of BMDCs for qPCR analyses (a) and co-culture experiments (b). The schematics in a and b were created using BioRender. (c) Cd80 mRNA expression after synchronization of immature BMDCs from WT (n = 15 mice) and Bmal1^−/− (n = 4 mice) mice without LPS maturation, Cosinor analysis. (d) qPCR of LPS-matured BMDCs at different times after synchronization; n = 9 mice from 2 independent experiments, one-way ANOVA. (e) Predicted binding regions of BMAL1 to the Cd80 gene using Eukaryotic Promoter Database with a cutoff P-value of 0.001. (f) Chromatin immunoprecipitation (ChIP) of BMAL1 binding the promoter of Per2 of BMDCs after synchronization; n = 3 mice, from 2 independent experiments, two-way ANOVA. All data are represented as mean ± SEM, ns, not significant.

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