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. 2022 Dec 5;614(7946):136–143. doi: 10.1038/s41586-022-05605-0

Extended Data Fig. 3. Leukocyte populations in skin and dLN.

Extended Data Fig. 3

(a) Flow cytometry gating strategy of skin myeloid populations. (b-c) Number (b) and proportion (c) of leukocytes at the tumour engraftment site 4 h after B16-F10-OVA engraftment; n = 8 mice from 2 independent experiments, unpaired Student’s t-test. Leu, leukocyte; N, neutrophils; EOS, eosinophil; IM, inflammatory monocytes; NK, natural killer cells. (d) Flow cytometry gating strategy of DC subsets in draining lymph nodes (dLN). (e-i) Leukocytes, T cell (e-f) and DCs (g-h) in the dLN, 24 h after tumour engraftment (5×105 B16-F10-OVA); n = 8 mice, from 2 independent experiments, unpaired Student’s t-test. (i) Gating of anti-mouse H-2Kb bound to SIINFEKL+ DCs in the B16-F10-OVA model. All data are represented as mean ± SEM, all t-tests are two-tailed.

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