Extended Data Fig. 11. SynCAMs function in primary cells: mesenchymal stem cells (MSCs), dermal fibroblasts, and iPSC derived smooth muscle cells (linked to main Fig. 4).

Maximum projection of 20x confocal microscopy images of αGFP and GFP synCAMs (with ICAM-1 ICD) or corresponding tether (no ICD) expressed in MSCs (a, scale bar = 10 µm) primary dermal fibroblasts (b, scale bar = 20 µm) or iPSC derived SMCs (c, scale bar = 20 µm). αGFP cells were also labelled with mCherry; GFP cells were also labelled with BFP. Representative images are shown of three independent replicates. In both cell types, the GFP-tether is diffusely spread throughout the cell. In contrast, the GFP-synCAM is strongly enriched atheterotypic cell–cell interfaces (white arrows). When cells expressing GFP-synCAMs are plated without their partner cells, the GFP is diffusely distributed throughout the cell.