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. 2023 Jan 5;24(2):295–308. doi: 10.1038/s41590-022-01386-w

Extended Data Fig. 3. Gating strategy for the isolation of Th cell subsets from the blood.

Extended Data Fig. 3

Fresh PBMCs from healthy donors were enriched for CD4 expression by MACS before FACS for CD45RA Th cell subsets according to their differential expression of the chemokine receptor markers CCR6, CCR4 and CXCR3. A representative gating strategy and the percentage of Th-cell subsets in the respective gates for 33 healthy donors are shown (mean ± SEM).