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. 2023 Jan 18;614(7946):125–135. doi: 10.1038/s41586-022-05620-1

Extended Data Fig. 10. Assessment of strain identity thresholds.

Extended Data Fig. 10

A) Centred nGD (normalised phylogenetic distance divided by the median nGD of the phylogenetic tree) used as a threshold for strain identity (corresponding to the percentiles of interindividual distributions) by strain definition used, for the 646 SGBs profiled in stool samples. The different percentiles do not result in significant differences in nGD values (Kruskal-Wallis test, Chi2=2.34, P = 0.31). Boxes: lower and upper quartiles, middle line: median; whiskers: 1.5 × IQR. B) Distribution of centred nGD thresholds (normalised phylogenetic distance divided by the median nGD of the phylogenetic tree) by phylum, showing lack of statistically-significant association (Kruskal-Wallis test, Chi2=6.6, P = 0.25). Boxes: lower and upper quartiles, middle line: median; whiskers: 1.5 × IQR. C) Strain identity thresholds (percentile of interindividual nGD distribution) calculated for each of the SGBs prevalent in longitudinal datasets (N = 145 SGBs profiled in at least 50 same-individual pairs) calculated on single datasets compared to the threshold used in the study (determined on all samples).