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. 2023 Jan 20;33(2):147–164. doi: 10.1038/s41422-022-00760-5

Fig. 2. hEnSC transplantation modulates local immune system in ALF livers.

Fig. 2

a Cell counts of the monocyte/MoMF/Kupffer cell population isolated from rat livers 24 h post transplantation. b FACS analysis for CD68+CD163+ M2-like cells in the monocyte/MoMF/Kupffer cell populations isolated from ALF rat livers 24 h post transplantation; right panel: statistical differences among the groups calculated from the data of three independent experiments. c qRT-PCR showing expression of the indicated genes in the monocyte/MoMF/Kupffer cell samples isolated from PBS-treated ALF (ALF) and hEnSC-transplanted (hEnSC) rats; values were determined relative to TATA-binding protein (Tbp); n = 3 independent animals for ALF or hEnSC groups. d Cell counts of the lymphocytes isolated from rat livers 24 h post transplantation. e FACS analysis for CD4+IFNγ+ Th1 and CD4+IL17A+ Th17 cell populations in the lymphocytes isolated from ALF rat livers 24 h post transplantation; middle and right panels: statistical differences among the groups calculated from the data of three independent experiments on Th1 and Th17 populations. f FACS analysis for CD4+CD25+FOXP3+ Treg cells on the lymphocytes isolated from ALF rat livers 24 h post transplantation; right panel: statistical differences among the groups calculated from the data of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (unpaired two-tailed Student’s t-tests). Error bars represent SD. See also Supplementary information, Figs. S6, S7.