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. 2023 Jan 20;33(2):147–164. doi: 10.1038/s41422-022-00760-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Upper panel: schematic diagram of in vitro migration assay; lower panel: representative fluorescence images captured by live imaging system showing the movement of EGFP-hEnSCs and DiI-Ac-LDL-labeled MoMFs/Kupffer cells. b Left: schematic diagram of intravital microscopy used to monitor in vivo interactions between hEnSCs and MoMFs/Kupffer cells; right: representative fluorescence images captured by live confocal microscope showing the interactions between EGFP-hEnSCs (pink arrows) and F4/80-PE-stained MoMF/Kupffer cells (blue arrows), and the interactions between EGFP-hESCs (yellow arrows) and F4/80-PE-stained Kupffer cells (blue arrows). c qRT-PCR showing expression of the indicated ISGs in the monocyte/MoMF/Kupffer cell populations cocultured with or without hEnSCs. The monocyte/MoMF/Kupffer cell populations were isolated from ALF rat livers at 24 h post D-GalN treatment, and were cocultured for 3 days with an initial seeding ratio of 1:1 in the presence or absence of rat recombinant IFNγ. Values were determined relative to Tbp. n = 3 independent samples for each group. Tukey’s multiple comparisons test was used to calculate significance between groups. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. See also Supplementary information, Figs. S12, S13, Videos S2–S4.