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. 2023 Jan 20;33(2):147–164. doi: 10.1038/s41422-022-00760-5

Fig. 5. hEnSC-secreted CST1 is responsible for the inhibition of IFNγ signaling.

Fig. 5

a Heatmap of the top 10 DEGs identified by scRNA-seq (SMART-seq2) across the cell populations of hEnSCs, hESCs, hESC-derived hepatoblasts or hepatocytes, showing the exclusive expression of CST1 in hEnSCs. b qRT-PCR showing expression of CST1 in the cell lines of H9 hESCs (hESC), CST1-overexpressing H9 hESCs (CST1+ hESC), human umbilical mesenchymal stem cells (hMSC P4), H9 hESC-derived EGFP-hEnSCs (hEnSC), and hEnSCs with shRNA-mediated knockdown of CST1 (CST1-shRNA hEnSC). Values were determined relative to TBP; n = 3 independent samples for each group. c qRT-PCR showing expression of indicated ISGs in monocyte/MoMF/Kupffer cell populations that were isolated from ALF rat livers 24 h post D-GalN administration and cultured in vitro for 3 days in the presence or absence of CST1 protein (500 ng/mL) and/or recombinant rat IFNγ (100 ng/mL). Values were determined relative to Tbp; n = 3 independent samples for each group. d Kaplan–Meier survival curves of the ALF rats transplanted with H9 hESCs (hESC), CST1-overexpressing hESCs (CST1+ hESC), rat primary hepatocytes, hEnSCs, hEnSCs with shRNA-mediated knockdown of CST1 (CST1-shRNA hEnSC) and hEnSCs + anti-rat IL10 antibody (1 mg/rat) (hEnSC + anti-rat-IL10). e Serum levels of ALT, AST, NH3 and PT detected in the indicated groups as shown in d. n = 3 for each group. Blood samples were collected 3 days post D-GalN administration. f Kaplan–Meier survival curves of the ALF rats transplanted with different doses of CST1 protein. 1 mg/200 μg/40 μg CST1: 1 mg or 200 μg or 40 μg CST1 protein per rat; Buffer control: PBS + 10% glycol. g Serum levels of ALT, AST, NH3 and PT detected in the indicated groups as shown in f. n = 3 for each group. Blood samples were collected 3 days post D-GalN administration. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Tukey’s multiple comparisons test was used in b, c, e and g, and Log-rank (Mantel-Cox) test was used in d and f. See also Supplementary information, Fig. S14.