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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Neurobiol Aging. 2022 Dec 22;123:92–97. doi: 10.1016/j.neurobiolaging.2022.12.007

Figure 1.

Figure 1.

Young adult mice with intact memory in the novel object location task had transiently increased neuronal excitability in CA1 ensemble cells after learning. (A) Schematic of novel object location task. Young mice had higher DI in test session vs. training session (paired t-test, p=0.03, N=8). (B) Doxycycline (dox) controls the tagging of ensemble cells in CA1. Left: representative images of eYFP+ in CA1 in mice on and off dox. Right: percentage of cells tagged in both groups (unpaired t-test, p=0.01, N=5 mice/condition). (C) In young adult mice (3–6 months, N=7 for 5 hours, N=6 for 7 days), neuronal excitability of CA1 ensemble cells increased 5 hours after context exploration compared to non-ensemble cells (two-way RM ANOVA, F1,20=5.68, p=0.03, n=10 cells eYFP-, 12 cells eYFP+). Neuronal excitability of ensemble cells returned to the same level as non-ensemble cells 7 days after (two-way RM ANOVA, F1,21=0.32, p>0.05, n=11 cells eYFP-, 12 cells eYFP+). (D) In young mice (N=7 for 5 hours, N=6 for 7 days), the resting membrane potential (RMP) of ensemble cells was not different from that of non-ensemble cells 5 hours (unpaired t-test, p>0.05, n=10 cells eYFP-, 12 cells eYFP+) or 7 days (unpaired t-test, p>0.05, n=11 cells eYFP-, 12 cells eYFP+) after learning.