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. Author manuscript; available in PMC: 2024 Jan 31.
Published in final edited form as: Circulation. 2022 Nov 23;147(5):388–408. doi: 10.1161/CIRCULATIONAHA.122.059062

Figure 1. 25-hydroxycholesterol accumulates in human coronary atherosclerotic plaque and CH25H is highly express in human and mouse inflammatory plaque macrophages.

Figure 1.

A, Representative hematoxylin, and eosin (H&E) staining (upper panels) and CD68 (macrophage marker) immunofluorescence (lower panels) of atherosclerotic plaques isolated from human coronary arteries. Scale bar: 1 mm. B, 25-hydroxycholesterol quantification in atherosclerotic plaques isolated from human coronary arteries. Data were analyzed by one-way ANOVA, post-hoc Bonferroni’s multiple comparison test (n= 6 mild plaques, 6 moderate plaques and 4 severe plaques). C, Violin plots showing CH25H expression among different macrophages clusters presents in human plaque tissue48. D, Violin plot showing the expression of Ch25h withing the indicated macrophage clusters from leukocyte isolated from Ldlr−/− aortic tissue10 of mice fed a WD for 12 weeks.