TABLE 2.

Top regulated genes by acute experimental stress in the colorectal mucosa of healthy volunteers

Gene symbol	Gene/protein name	Fold change	FDR	Main function of gene product
PCK1	Phosphoenolpyruvate carboxykinase	1.83	0.050	Acts as the rate‐limiting enzyme in gluconeogenesis and controls levels of metabolic intermediates in the citric acid cycle. Regulates formation and maintenance of memory CD8(+) T‐cells.
CR2	Complement C3d receptor 2	−2.18	0.021	Receptor for complement C3, on human B‐cells a and T‐cells. Participates in B‐cell activation.
CXC13	C‐X‐C motif chemokine 13	−1.97	0.050	Chemotactic for B‐lymphocytes but not for T‐lymphocytes, monocytes and neutrophils. Binds to chemokine receptor CXCR5.
CCL21	C‐C motif chemokine 21	−1.82	0.029	Chemotactic for activated T‐cells, B‐cells, and dendritic cells. Binds to atypical chemokine receptor CCR7.
MS4A1	Membrane spanning 4‐domains A1	−1.78	0.049	B‐lymphocyte‐specific membrane protein that plays a role in the development, differentiation, and activation of B‐lymphocytes.
BANK1	B cell scaffold protein with ankyrin repeat	−1.70	0.029	Involved in B‐cell receptor (BCR)‐induced Ca(2+) mobilization from intracellular stores.
CCL19	C‐C motif chemokine ligand 19	−1.62	0.022	Potent chemotactic activity for T‐cells and B‐cells. Binds to chemokine receptor CCR7 and ACKR4.
DOUX2	Dual oxidase 2	−1.59	0.050	Generates hydrogen peroxide; playing role in Lactoperox‐idase‐mediated antimicrobial defense at mucosal surface.
TCLA1	T cell leukemia/lymphoma 1 family AKT coactivator A	−1.51	0.050	Phosphorylation and activation of AKT1, AKT2 and AKT3. Stabilizes mitochondrial membrane potential and promotes cell survival.
CD22	CD22 molecule	−1.51	0.044	Mediates B‐cell–B‐cell interactions. Involved in localization of B‐cells in lymphoid tissues and regulation of B‐cell antigen receptor signaling.

Note: Gene symbol and name for the up‐ and down‐regulated genes and their fold‐change expression respect to basal state are indicated. Data are based on analysis of mRNA expression in seven individuals in control and stress sessions, respectively. p values were adjusted to obtain strong control over the “false discovery rate” (FDR) using the Benjamini and Hochberg method. Main protein functions from The Human Protein Atlas (https://www.proteinatlas.org). PCK1 was the only up‐regulated gene, all others were significantly down‐regulated.