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Dynamics of antigen bound to BLPs after oral and nasal administration. The luciferase activities in lymphoid tissues (PPs, mLN, NALT and spleen) at 2 or 6 h after oral or nasal administration of 2 × 109 BLP-NanoLuc. Data were expressed as the mean ± SEM of whole tissue (n=6). Each symbol represents an independent mouse. Dotted line indicates the detection limit. *, p<0.05; ***, p<0.001; and ****, p<0.0001; one-way ANOVA followed by Dunnett’s multiple comparison test.
