Figure 1.

FRET-based binding assays. (A) FRET increased with increasing protein expression. Example binding curve for SLN ALN with hyperbolic function of best fit shown in red. (B) Examples of different combinations of micropeptides that showed high or low FRET_max and dissociation constants (K_Ds). Data are mean FRET pooled from cells expressing a similar level of protein. X/Y standard deviation denoted using brackets. (C) The apparent homo-oligomerization affinities of micropeptide for human protein sequences (measured here) were similar to values previously obtained with mouse sequences. The gray dotted line represents 1:1 correspondence.