Figure 2.

SEC-SAXS results for apo-MexR. (A) The final averaged SAXS profile of MexR thought SEC elution peak. In purple are the experimental data and in black the DAMFILT curve obtained from the DAMFILT volume shown in (D) $\left({\chi }^2=1.30\right)$; curves show the best fit of the theoretical scattering curves of MexR structures 1LNW:AB (red, ${\chi }^2=1.60$), 1LNW:CD (blue, ${\chi }^2=1.41$); 1LNW:EF (yellow, ${\chi }^2=1.58$), and 1LNW:GH (green, ${\chi }^2=1.47$). Insert: the corresponding Guinier plot and linear fit, where the R_g was assed at 23.2 Å within the sR_g limit of 0.2 < sR_g < 1.3. (B) Dimensionless Kratky plot of the SAXS profile of apo-MexR. (C) P(r) versus $r$ profile calculated from the SAXS data. (D) DAMFILT ab initio model (beads) superposed with 1LNW:AB, CD, EF, and GH structures in ribbon representation, colored as in (A). To see this figure in color, go online.