Ensemble-averaged values calculated from the SAXS-reweighted CALVADOS ensembles, compared with the determined by PFG NMR diffusion (error bars represent the standard error from fitting the NMR data). We tested four approaches to calculate the from atomic coordinates: the -dependent Nygaard equation (, in blue), the Kirkwood-Riseman equation (, in orange), HullRad (, in green), and the Nygaard correction to the Kirkwood-Riseman equation (, in red). To see this figure in color, go online.