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. 2023 Jan 19;13:1087689. doi: 10.3389/fimmu.2022.1087689

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Copyright © 2023 Luo, Zhou, Zhang, Zhang, Long, Lin, Yang, Duan, Yang, Yang, Che, Yang, Guo, Zi, Ouyang, Yang, Zeng and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional characterization of eNK-EXO in vitro. (A) Expression of eNK-EXO surface markers CD56, CD16, CD69 and CD107a by NanoFCM. Red dots represent positive signals. (B) Western blot analysis of CD56 (122KD), perforin (61KD) and GranzymeB (40KD) expression. NK cell lysate was used as control. (C) CCK-8 assay results of eNK-EXO against SKOV3, COC1/DDP and IOSE80 cells (n=3, mean ± SEM, t-test, *p< 0.05, **p< 0.01). (D, E) EdU assay results of eNK-EXO against SKOV3 (20×, scale bar = 100 µm). Red signals represent newly proliferating cells, whose proportion was quantified and shown (n=3, mean ± SEM, ***p< 0.001) (E).