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. 2023 Jan 19;13:1087689. doi: 10.3389/fimmu.2022.1087689

Figure 3.

Figure 3

Cellular uptake results of eNK-EXO by OC cells. (A) eNK-EXO uptake by SKOV3 cells. SKOV3 cells were stained blue by DAPI and eNK-EXO were stained green by PKH67. (B) eNK-EXO uptake quantified by green fluorescence intensity (10×, Scale bar=200 µm; 100×, Scale bar=40 µm, n=5, mean ± SEM, ***p< 0.001). (C) eNK-EXO uptake by SKOV3 in the presence of anti-CD63 or anti-CD81. SKOV3 cells were stained blue by DAPI and eNK-EXO were stained green by PKH67 (20×, scale bar=100 µm). (D) eNK-EXO uptake quantified by green fluorescence intensity (n=4, Mean ± SEM, t-test, ***p< 0.001). (E) Uptake of PKH67-labeled eNK-EXO in the co-culture system of IOSE80 cells and DiR-labeled SKOV3 cells (10×, scale bar=200 µm; 10×, scale bar = 40 µm).