TABLE 2.

Kinetic constants from the recent literature^a

Culture	Substrate	Specific affinity (aos) (liters mg of cells−1 h−1)c	Maximumal velocity (Vmax) (mg of S mg of cells−1 h−1)	Km (μg liter−1)	KA (μg liter−1)	Reference
Cycloclasticus oligotrophus	Toluene	47.4	1.2	10	1.3	Unpublished data
Escherichia coli	Glucose	7.3	4.8	50	≥50	38
Pseudomonas sp. strain P-15	Phenanthrene	1.2	0.06	>400	400	60
Seawater, Resurrection Bayb	Leucine	0.83	0.006	>2.6	2.6	Unpublished data
Pseudomonas sp. strain T2	Toluene	0.32	0.14	44	44	53
Rumen isolate SR	Arginine	0.30	0.41	13,900	1,044	63
Escherichia coli	Leucine	0.21	0.010	1,570	∼260	69
Sphingomonas sp. strain RB 2256	Glucose	0.2				56
Corynebacterium glutamicum	Glycine betaine	0.11	0.25	1,015		23
Sphingomonas sp. strain RB 2256	Alanine	0.06	0.029	440	440	56
Mixed methanotroph culture	Trichloroethylene	0.06	0.51	7,990		17
Desulfovibrio G-11 and syntropic benzoate isolate	Benzoate	0.025	0.045	1,732		66
Corynebacterium glutamicum	Tyrosine	0.02	0.011	543		67
Escherichia coli	Gluconate	0.017	0.08	4,900		64
Pseudomonas sp. strain B13 (chemostat)	3-Chlorobenzoate	0.016	0.032	6,768		61
Peter Lake	Leucine	0.016				47
Escherichia coli	Leucine (repressed)	0.008	0.009	7,000	500	69
Saccharomyces cerevisiae	Glucose	0.0016	0.54	5.6 × 106	1.1 × 105	64
Cycloclasticus oligotrophus	Acetate	0.0016	0.13	20,000	20,000	Unpublished data
Lactobacillus brevis 367	Trimethyl-β-galactoside	0.0010	0.14	1.1 × 105		28
Escherichia coli	p-Nitrophenyl phosphate	0.0072	0.65	44,630	149,000	40
Penicillium chrysogenum	Phenylacetic acid	3.3 × 10−4	>6.1	>5 × 106		27
Vibrio parahaemolyticus	Glucose	2.6 × 10−4	0.027	1.0 × 105		55
Halobacterium saccharovorum	Glucose	2.8 × 10−4	0.07	1.4 × 105	28,000	59
Seawater, Resurrection Bayc	Toluene	0.85 × 10−6				10
Escherichia coli K-12	Glycine	1 × 10−6	2 × 10−5	2.0 × 105		24

^aConversion factors used: 400 mg/liter (wet wt) OD⁻¹ (30), 3 mg of cells (wet wt) (mg dry wt)⁻¹, 1.9 mg of cells (dry wt) mg of protein⁻¹. 

^bBiomass is taken as 53% of the total population which used leucine, as determined by autoradiography, and corrected for the 0.8-μg-liter⁻¹ ambient leucine present (12). 

^cBiomass is taken as the total bacterial population, which was 7% toluene oxidizers. The specific affinity was corrected for saturation by using to the kinetic constants shown (constants were obtained with Cycloclasticus oligotrophus [data not shown]).