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Production of the clinical grade mesenchymal stem cells from umbilical cord tissue in good manufacturing practice (GMP) laboratory.
| Wash the umbilical tissue three times with normal saline ↓ |
| Divide the tissue into 2 cm pieces ↓ |
| Remove 2 arteries 1 vein ↓ |
| Cut the tissue into 1–2 mm3 pieces ↓ |
| Place in flasks with culture method ↓ |
| Follow up ↓ |
| Follow the cellular structures around the tissue, passage when confluent ↓ |
| Freeze/Send |
