表2.
不同分析方法获得的肝素或低分子量肝素的二糖组成
| No. | Composition | SAX- UVHep[63] |
SAX- SECHep[65] |
SAX- SECEno[66] |
SAX- SECNadro[66] |
IPRP- MSEno[70] |
HILIC- MSNadro[72] |
HILIC- MSEno[60] |
||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ΔU-GlcNAc | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 2 | ΔU-GlcNS | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 3 | ΔU-GlcNAc，6S | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 4 | ΔU2S-GlcNAc | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 5 | ΔU-GlcNS，6S | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 6 | ΔU2S-GlcNS | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 7 | ΔU2S-GlcNAc，6S | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 8 | ΔU2S-GlcNS，6S | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 9 | ΔGalA-GlcNS | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 10 | ΔGalA-GlcNS，6S | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 12 | IdoA2S-GlcNS | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 13 | U2S-GlcNS or U-GlcNS，6S | 0 | 0 | 1 | 0 | 0 | 0 | 0 | ||
| 14 | U2S-GlcNAc or U-GlcNAc，6S | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 15 | IdoA2S-GlcNS，6S | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 16 | ΔU2S-GlcN，6S | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 17 | ΔU2S-GlcN | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 18 | ΔU-GlcN，6S | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 19 | ΔU-GlcN | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
| 20 | ΔGlyser | 1 | 1 | 1 | 1 | 0 | 1 | 1 | ||
| No. | Composition | SAX- UVHep[63] |
SAX- SECHep[65] |
SAX- SECEno[66] |
SAX- SECNadro[66] |
IPRP- MSEno[70] |
HILIC- MSNadro[72] |
HILIC- MSEno[60] |
||
| 21 | ΔGlyserox1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 22 | ΔGlyserox2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 23 | Other non-endogenous derivatives ΔISO3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 24 | Tetrasaccharide A ΔIS-I2SO3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 25 | Tetrasaccharide B ΔI2SO3-Isid | 1 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 26 | ΔU-GlcNAc，6S-GlcA-GlcNS，3S | 1 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 27 | ΔU-GlcNAc，6S-GlcA-GlcNS，3S，6S | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 28 | ΔU-GlcNS，6S-GlcA-GlcNS，3S，6S | 1 | 1 | 1 | 0 | 0 | 1 | 1 | ||
| 29 | ΔU2S-GlcNAc，6S-GlcA-GlcNS，3S，6S | 1 | 0 | 0 | 0 | 0 | 1 | 0 | ||
| 30 | ΔU2S-GlcNS，6S-GlcA-GlcNS，3S，6S | 1 | 0 | 0 | 0 | 0 | 1 | 0 | ||
| 31 | GlcNS-IdoA2S-GlcNS，6S or GlcNS， | 0 | 0 | 1 | 0 | 0 | 0 | 0 | ||
| 6S-IdoA2S-GlcNS | ||||||||||
| 32 | GlcNS，6S-IdoA2S-GlcNS，6S | 1 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 33 | ΔU-GlcNS，6S-HexA | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 34 | ΔU2S-GlcNAc，6S-HexA | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
| 35 | ΔU2S-GlcNS，6S-HexA | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 36 | ΔU2S-GlcNS，6S-HexA2S | 0 | 0 | 1 | 0 | 0 | 1 | 1 | ||
| 37 | ΔU2CS-GlcNS，6S | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 38 | GlcNS，6S-U2CS-GlcNS or GlcNS-U2CS-GlcNS，6S | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 39 | ΔUA2S-GlcNS，6S-GlcA-2，3-anhydro-GlcNS | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 40 | ΔUA-GlcNS-HexA2S，3S-GlcNS | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
| 41 | 3-O-S Δdp2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
| 42 | 3-O-S Δdp4（3S，1Ac） | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
| 43 | 3-O-S Δdp4（4S，0Ac） | 0 | 1 | 1 | 0 | 0 | 1 | 0 | ||
| 44 | 3-O-S Δdp4（4S，1Ac） | 0 | 2 | 0 | 0 | 0 | 0 | 0 | ||
| 45 | 3-O-S Δdp4（5S，0Ac） | 0 | 3 | 0 | 0 | 0 | 0 | 0 | ||
| 46 | ΔU- Mnt6S | 0 | 0 | 0 | 1 | 0 | 1 | 0 | ||
| 47 | ΔU2S- Mnt6S | 0 | 0 | 0 | 1 | 0 | 1 | 0 | ||
| 48 | GlcNS，6S-U2S-Mnt6S | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 49 | ΔU2S-GlcNS-U-Mnt6S | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
| 50 | ΔU2S-GlcNAc-U2S-Mnt6S | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 51 | ΔU2S-GlcNS-U2S-Mnt6S | 0 | 0 | 0 | 1 | 0 | 1 | 0 | ||
| 52 | ΔU2S-GlcNS，6S-U2S-Mnt6S | 0 | 0 | 0 | 1 | 0 | 1 | 0 | ||
| 53 | MntΔdp5（5S，1Ac） | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 54 | MntΔdp6（6S，1Ac） | 0 | 0 | 0 | 1 | 0 | 0 | 0 | ||
| 55 | ΔU-1，6-anhydroGlcNS | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
| 56 | ΔU-1，6-anhydroManNS | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
| 57 | ΔU2S-1，6-anhydroHexNS | 0 | 0 | 0 | 0 | 2 | 0 | 1 | ||
| 58 | GlcNS，6S-U2S-1，6-anhydroHexNS | 0 | 0 | 1 | 0 | 0 | 0 | 0 | ||
| 59 | ΔU2S-GlcNS，6S-U2S-1，6-anhydroManNS | 0 | 0 | 0 | 0 | 1 | 0 | 1 | ||
Hep：heparin sodium；Eno：enoxaparin sodium；Nadro：nadroparin calcium；Mnt：2，5-AM. ol；SEC：size-exclusion chromatography；HILIC：hydrophilic interaction chromatography；ΔU2S-GlcNAc，6S：unsaturated disaccharide with 2-O-sulfated uronic acid and N-acetylated，6-O-sulfated glucosamine；3-O-S Δdp4（3S，1Ac）：unsaturated tetrasaccharide with 3-O-sulfated glucosamine（total three sulfo groups，one acetyl group）；1：detected；0：no detected.