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. 2023 Feb 1;43:8. doi: 10.1186/s41232-022-00254-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6. — VEGF-A is expressed in the VZ where NSPCs are confined in the developing cortex. A Vegf-A mRNA expression in the E11.5 and 14.5 brain was detected by in situ hybridization. Right panels indicate magnification of boxed area. Scale bar = 50 μm. B Sense probe was used as negative control. Right panels indicate magnification of boxed area. Scale bar = 50 μm. C Immunohistochemistry by anti-Hif-1a antibody in the E14.5 cortex. The center and right panels indicate magnification of boxed area in the left panel. The center panel shows single color image of Hif1-a. Scale bar = 50 μm. LV, lateral ventricle.