FIG. 9.

Effect of diethanolamine on uphill K⁺ extrusion and the internal pH of E. hirae. The K⁺ movement (A) and the internal pH change (B) of the cells at pH 9.0 were monitored in a high-K⁺ buffer (250 mM K⁺). Solid symbols, glucose added; open symbols, no glucose. The reaction was initiated by addition of diethanolamine as indicated by the arrows. Symbols: ○, no addition; •, 20 mM diethanolamine; ▴, 50 mM diethanolamine; □, control (in a buffer containing 50 mM diethanolamine but not glucose). Reprinted from reference 113 with permission of the publisher.