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. 2023 Jan 18;57(4):1731–1742. doi: 10.1021/acs.est.2c07281

Figure 4.

Figure 4

MSC immunosuppressive capabilities are only slightly reduced following exposure to PCB mixtures. (A) Timeline of the PBMC-MSC coculture. Immunosuppressive capabilities of MSCs were assessed by measuring the dilution of CFSE dye in PBMCs after coculture with MSCs that had been pre-exposed for 48 h to (B) Aroclor 1016, (C) Aroclor 1254, or (D) Cabinet Mixture. The percent of PBMCs that proliferated was quantified to assess MSC immunosuppressive potency after exposure to (E) Aroclor 1016, (F) Aroclor 1254, or (G) Cabinet Mixture. Bars are mean +/– SD of n = 3 independent adipose donors. Ordinary one-way ANOVA, * designates a significant difference (p < 0.05) between the indicated group and vehicle control (DMSO) pre-exposed MSCs after Dunnett multiple comparison corrections. Cell figures were adapted from https://smart.servier.com/ and licensed under CC-BY 3.0.