TABLE 2.

Information for the ORFs sequenced from P. denitrificans and P. versutus

Characteristic	P. denitrificans	P. versutus
% G+C from sequence	65.63	64.90
% G+C (experimental)a	66.5	66.8
Total DNA sequencedb	166,864	16,092
% of genome sequencedc	4.17	0.81
No. of ORFs identified	162	19
No. of ORFs (defined function)d	129	13
Total length of ORFs (bp)e	115,489	11,718

^aExperimental data taken from reference 145. 

^bThe total DNA sequenced includes all known strains of P. denitrificans, including strain GB-17. 

^cThe total length of both genomes is taken to be 4,000 kbp, the size estimated for P. denitrificans by pulsed-field gel electrophoresis (323). The duplicate sequencing of the cycA (cytochrome c₅₅₀) locus has been taken into account, but all other sequences are taken to be unique and nonoverlapping. 

^d“Defined function” is taken to mean either that the gene product has had its biochemical effect identified experimentally or that the gene product has significant identity to a protein from another organism. 

^eThe shorter total length of the ORFs compared to the total DNA sequenced does not reflect large intergenic regions in Paracoccus; rather, it reflects the fact that sequencing targeted to specific genes leaves regions to the 3′ and 5′ of the loci that are not of sufficient length to have the definition “ORF” applied to them.