TABLE 2.

Pairwise comparison of the amino acid sequences of inserts of multimodular PBPs

Subclass	PBPa	Similarity (%)
A2	Eco1b D88-D198/Cfr1b D88–D198	91
	/Hin1b D44–E150	32
B1	Efam5 E33-R168/Ehi3r E38–R173	97
	/Ehi5 E38–R173	65
	/Efas5 E43–R175	35
	/Bsu3 D25–R159	27
	Ehi5 E38–R173/Efas5 E43–R175	37
	/Bsu3 D25–R159	28
	Efas5 E43–R175/Bsu3 D25–R159	23
	Sau2a D27–R151/Ssc2 E31–R150	71
	/Efam5 E33–R168	17
	/Ehi3r E38–R173	17
	/Ehi5 E38–R173	17
	/Efas5 E43–R175	17
	/Bsu3 D25–R159	18
A1	Eco1a N526–K716/Hin1a N501–K717	48
	/Pae1a N522–K695	31
	Hin1a N501–K717/Pae1a N522–K695	31
A1	Nme1/Ngo1 Q298–L428/Eco1a Q296–I432	25
	/Hin1a Q286–L408	26
	/Pae1a Q296–L428	26
	/Aaemrca Q291–I399	21
	/Bbu3 Q316–I428	6
	Eco1a Q296–I432/Hin1a Q286–L408	45
	/Pae1a Q296–L428	40
	/Aaemrca Q291–I399	16
	/Bbu3 Q316–I428	4
	Hin1a Q286–L408/Pae1a Q296–L428	32
	/Aaemrca Q291–I399	14
	/Bbu3 Q316–I428	9
	Pae1a Q296–L428/Aaemrca Q291–I399	18
	/Bbu3 Q316–I428	10
	Aaemrca Q291–I399/Bbu3 Q316–I428	15

^aThe PBP codes are given in Table 1. PBPs Nme1 and Ngo1 have 98% identity. 

^bSimilarity is expressed as percent identity.