Fig. 2.

Gene expression profiling of BodoOBP10. (A) RT-qPCR analysis in different developmental stages: Eggs; Larvae; Pupae; Female (newly emerged and virgin); Male (newly emerged and virgin). (B) RT-qPCR analysis in different tissues: FA: Female antenna; MA: Male antenna; Head; Abdomen; Carcass: leg + wing + thorax. The expression levels were estimated using the 2^−ΔΔCt method. The expression level in eggs was used as a standard to compare expression levels among developmental stages, and the expression level in male antennae was used as a standard to compare expression levels among tissues. One-way analysis of variance (ANOVA) was used for analyzing differences in expression patterns using the LSD method. Data are normally distributed. SPSS 20.0 (IBM Corp., Armonk, NY, USA) was used for statistical analysis and the values are means + SE; means with different letters are significantly different (P < 0.05).