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. 2022 Nov 28;119(49):e2212548119. doi: 10.1073/pnas.2212548119

Fig. 2.

Fig. 2.

Expansion of the fetal layer drives enhanced protection in dirty mice. (A) Approach to timestamp the fetal and adult layers in clean and dirty mice. (B) The proportion of fetal- and (C) adult-derived CD8+ T cells in 8-wk-old clean and dirty mice. (D) Schematic showing the approach to deplete the fetal layer. (E) Pathogen burden in clean and dirty mice lacking the fetal layer of CD8+ T cells. (F) Schematic showing the approach to prevent the adult layer from forming. (G) Pathogen burden in clean and dirty mice lacking the adult layer of CD8+ T cells. Data for B and C are ±SEM and are pooled from two independent experiments (n = 3–4 mice/group). Data for E and G are mean ± SEM and are pooled from two independent experiments (n = 3–4 mice/group). Statistical significance for B and C was determined by Student’s t test. Significance for E and F was determined by a one-way ANOVA with a Tukey multiple comparison posttest (ns = not significant, *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.00005).