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. 2022 Dec 2;119(49):e2216712119. doi: 10.1073/pnas.2216712119

Fig. 1.

Fig. 1.

DHX15 specifically associates with the SUGP1 G-patch. (A, Upper) Schematic representation of plasmid constructs (1 to 4) expressing FLAG-GST tandem tags, FLAG-GST-tagged SUGP1 (aa 543–645), its G574A-G582A mutant, and FLAG-GST-tagged SUGP1 (aa 607–645), respectively. (Lower) Each of the four constructs was transfected into HEK293T cells, followed by small-scale affinity purification using FLAG and GST tags. The purified proteins in eluates were resolved by SDS-PAGE, followed by silver staining. (B) HEK293T cells were transfected with construct 2 in (A), followed by large-scale affinity purification using FLAG and GST tags. The purified proteins were resolved by SDS-PAGE, followed by staining with QC Colloidal Coomassie Stain (Bio-Rad). (C) Aliquots of the purified proteins in (A) were resolved by SDS-PAGE, followed by western blotting. In all panels: M, Precision Plus Protein marker (Bio-Rad).