Fig. 4.
Measurement of energy balance and insulin resistance in adult mice fed regular chow. (A) Changes of body weight (BW), lean mass, and fat mass during aging between young and adult WT and KO mice on regular chow (n = 5 for each group). Time course metabolic patterns obtained during 72 h of analysis for (B) locomotor activity, (C) VO2, (D) energy expenditure, and (E) food intake (n = 8 for each group in panel B–E). (F) Plasma glucose and glucose infusion rate (GIR) and (G) insulin-stimulated whole-body glucose uptake (Rd) during hyperinsulinemic-euglycemic clamp study on regular chow at the age of 7 mo. (n = 5–6 for each group in panel F and G). All data are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 by two-way ANOVA with post hoc analysis. Statistical numbers are by two-tailed Student's t test.