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. 2022 Nov 28;119(49):e2213628119. doi: 10.1073/pnas.2213628119

Fig. 7.

Fig. 7.

GH-stimulated IGF-1 response after JAZF1 knockdown in Huh7 human hepatocytes. (A) mRNA expression of JAZF1 in Huh7 and HepG2 cells. (B) mRNA expression of JAZF1 after JAZF1-specific siRNA (siJAZF1) treatment in Huh7 cells. (C) Western blot for HNF4α, JAK-STAT-IGF-1 protein in control, and siJAZF1 cells after GH treatment ((50 ng/ml, 1 h), siRNA 30 pmol, and P-JAK2 (T1007, T1008) P-STAT5b (s731)). Normalized western blot data for (D) JAZF1, (E) HNF4α, and (F) IGF-1 protein expression in control and siJAZF1 cells after GH treatment. N = 3–4 for each group. All data are mean ± SEM. *P < 0.05 by one-way ANOVA with post hoc analysis. Statistical numbers are by two-tailed Student's t test. n.d., non-detected.