Fig. 6.

Coexpression of CA and Ci uptake enabled rubisco-dependent growth of CCMB1 E. coli in ambient air. Inspecting the ambient CO₂ growth data presented in Fig. 4 revealed that coexpression of CA and Ci transport (“Rub.+DAB+CA”) substantially improved rubisco-dependent growth of CCMB1 E. coli in ambient CO₂ concentrations. This effect was modest (≈0.1 OD units above the “Rubisco Alone” control) but reproducible, as indicated by end point data plotted on the inset. Curves are colored to match labels on the inset. See SI Appendix, Fig. S10 for statistical analysis.