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. 2022 Dec 2;119(49):e2204259119. doi: 10.1073/pnas.2204259119

Fig. 4.

Fig. 4.

PRDM9-Cas9 fusion protein produces increased HDR:indel ratios in different cell types. (A) HDR frequency measured by NGS at multiple genomic loci (C7, C9, and C10) in HEK293T cells transfected with PRDM9-Cas9 and sgRNA with ssODN templates that either include (hash pattern) or lack (solid pattern) a mutation at the PAM site. (B) HDR:indel ratio measured by NGS at multiple genomic loci (C7, C9, and C10) in HEK293T cells transfected with PRDM9-Cas9 and sgRNA with ssODN templates that either include (hash pattern) or lack (solid pattern) a mutation at the PAM site. (C) HDR frequency and HDR:indel ratio measured by NGS at multiple genomic loci (C7–C9) in HeLa cells transfected with PRDM9-Cas9 and sgRNA with an ssODN template. (D) HDR frequency and HDR:indel ratio measured by NGS at multiple genomic loci (C7–C9) in U2OS cells transfected with PRDM9-Cas9 and sgRNA with an ssODN template. For A–D, data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001, determined by Student’s two-tailed t test.