Table 1.
Statistics for data collection, map reconstruction, and model refinement
| Actin filament branch (EMD- 27962, (49) PDB 8E9B (50)) | |
|---|---|
| Data collection and map reconstruction | |
| Magnification | 36,657 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 50.07 |
| Defocus range (µm) (set/measured) | [−2.5, −1.5]/[−2.9, −0.9] |
| Pixel size (Å) | 1.364 |
| Symmetry | C1 |
| Initial particle images (no.) | 131,393 |
| Final particle images (no.) | 79,467 |
| Map analysis in reconstructed/modeled regions | |
| Resolution (Å) at 0.143 threshold, Relion | 3.53/3.47 |
| Sharpening B factor (Å2), Relion | −101.52/−74.09 |
| Resolution (Å) at 0.143 threshold, 3DFSC | 3.38/3.35 |
| Sphericity (out of one), 3DFSC | 0.963/0.966 |
| Map resolution range (Å) | 3.2–5.6 |
| Model Refinement | |
| Initial model used (PDB ID) | 1K8K/6DJO/6W17/AlphaFold |
| Model-to-map fit correlation | 0.77 |
| Model resolution (Å) at 0.5 threshold | 3.6 |
| Model composition | |
| Heavy atoms | 39,160 |
| Protein residues | 4,933 |
| Ligands (ATP/ADP/Mg2+) | 1/9/10 |
| B factors (Å2) | |
| Protein | 94.59 |
| Ligands | 87.65 |
| RMS deviations | |
| Bond lengths (Å) | 0.003 |
| Bond angles (°) | 0.711 |
| Geometry validation | |
| MolProbity score | 1.91 |
| Clashscore | 10.43 |
| rotamer/Cβ outliers (%) | 0.05/0.00 |
| Ramachandran plot | |
| Favored (%) | 94.89 |
| Allowed (%) | 5.11 |
| Disallowed (%) | 0.00 |