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. 2022 Dec 1;119(49):e2215124119. doi: 10.1073/pnas.2215124119

Fig. 3.

Fig. 3.

The syntaxin linker region (a.a. 147-197) and the Munc18-1 loop in the 3a helical hairpin domain (a.a. 314-326) are required for chaperoned SNARE assembly with different mechanisms. (A) Positions and conformations of the two regions in the template complex. (B) FECs associated with truncated syntaxin-1 (Syx Δ147-197) or Munc18-1 (M18 Δ314-326), or Munc18-1 with the loop replaced by the glycine- and serine-rich sequence (M18 GS314-326) in the presence of 1 µM Munc18-1 and 60 nM SNAP-25 in the solution. (C) Extension-time trajectories at constant mean force for Syx-VAMP conjugates cross-linked at X-8 or X-6.