Figure 1.

Proliferation of monocytic THP-1 depends on exogenous glutamine. THP-1 cells were cultured in the presence or absence of 2 mM glutamine (Gln). Cell yield (A) and cell viability (B) of human THP-1 cells were determined after 48 h using the CASY Cell Counter. Shown are median values and single data points. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (*p < 0.05). (C) Pictures taken on days 2 and 12 to document the morphology of THP‐1 cells; one representative picture is shown for each condition. Cell yield (D) and viability (E) of THP‐1 cells were analyzed every other day (mean ± SEM; n =3). Statistical significance was determined by two-way ANOVA and Bonferroni’s multiple comparison test (*p < 0.05; ***p < 0.001).