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. 2023 Jan 19;13:1120194. doi: 10.3389/fonc.2023.1120194

Figure 2.

Figure 2

Differentiation of monocytes to dendritic cells is independent of exogenous glutamine. Human dendritic cells (DCs) were differentiated from monocytes in the presence of FCS, GM-CSF, and IL-4, with or without glutamine (Gln) for 7 days. (A) Representative gating of the differentiated DC population in SSC and FSC on day 7. (B) Pictures taken on day 7 to document the morphology of DCs; one representative picture is shown for each condition. (C) Cell count and (D) viability of differentiated DCs were determined on day 7 using the CASY Cell Counter. Symbols represent individual donors; lines link data points from the same donor. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected). (E, F) Surface marker expression was analyzed by flow cytometry. Shown are median values and single data points. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected). (G) Cytokines (IL-12, IL-10, IL-6, and TNF) were determined by ELISA in supernatants of DCs stimulated with LPS (100 ng/mL) for 24 h. Symbols represent individual donors. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected).