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. 2023 Jan 19;13:1120194. doi: 10.3389/fonc.2023.1120194

Figure 3.

Figure 3

Human macrophages do not rely on exogenous glutamine. Human macrophages were differentiated from monocytes in the presence of human serum with or without glutamine (Gln) for 7 days. (A) Representative gating of the monocyte-derived macrophage population in SSC and FSC on day 7. (B) Pictures taken on day 7 to document macrophage morphology; one representative picture is shown for each condition. (C) Cell yield and (D) viability of differentiated macrophages were determined on day 7 using the CASY Cell Counter. Symbols represent individual donors; lines link data points from the same donor. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected). (E, F) Surface marker expression was analyzed by flow cytometry. Shown are median values and single data points. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected). (G) Cytokines (TNF and IL-6) were determined by ELISA in supernatants of macrophages stimulated with LPS (100 ng/mL) for 24 h. Symbols represent individual donors. Statistical significance was calculated using Wilcoxon’s matched pair signed rank test (no significance detected). (H) IL-6 was determined by ELISA in supernatants of macrophages stimulated with LPS (100 ng/mL) for 24 h. Symbols represent individual donors. Statistical significance was calculated using Friedman test with Dunn`s multiple comparison test (*p < 0.05).