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. 2023 Feb 2;18(2):e0280499. doi: 10.1371/journal.pone.0280499

Fig 4. In vitro activity of engineered EcN strains in the absence of pks.

Fig 4

A) N-myristoyl-D-asparagine (colibactin biomarker) levels in supernatants of parental strains SYNB1618 (dark blue) and SYNB1934 (pink), while no biomarker is detected in Δpks SYNB1618 and Δpks SYNB1934. Data are the mean ± SD of 3 samples after growth in LB for 18h. ND = not detected, (LLOQ) was 0.8μg/mL. B) SYNB1618 and SYNB1934 Phe metabolizing activity in vitro, before and after deletion of the pks island. Bar graph shows the mean ± SD (n = 3) rate of TCA production in SYNB1618 (dark blue), Δpks SYNB1618 (light blue), SYNB1934 (pink), and Δpks SYNB1934 (orange) using an in vitro assay of TCA production over 1h. C) SYNB8802 oxalate metabolism with and without the pks island. Oxalate metabolism by SYNB8802 (intact pks island; dark blue) and Δpks SYNB8802 (deleted pks island; pink) were measured over 1h. Mean ± SD of 3 samples at each time point are shown.