Fig 6. Kinetics and activity of WT EcN and Δpks EcN in nonhuman primates (NHPs).

(A) Fecal excretion of EcN strains in NHPs (n = 6 per group) following oral administration of a combined dose (1x10¹² CFU of each bacterial strain) of WT EcN (pink) or Δpks EcN (dark blue) over 120h, (LLOQ = 1.5x10² CFU/g feces). Data are presented as mean ± SEM. Statistical analysis was performed using 2-way repeated ANOVA followed by Sidak’s multiple comparison test (p>0.05). (B) Appearance of plasma D5-TCA (B) and urinary recovery of D5-HA (C) in NHPs (n = 12 per group) following oral administration of a single dose (1x10¹² CFU) of SYNB1934 (pink) or Δpks SYNB1934 (dark blue). Data are presented as mean ± SEM. Statistical analysis was performed using 2-way repeated ANOVA followed by Sidak’s multiple comparison test (B) and unpaired t-test with Welch’s correction (C).