Table 1. Strains list.
| Strain | Antibiotic resistance | Genotype | Accession number |
|---|---|---|---|
| WT EcN | Streptomycin | Escherichia coli Nissle 1917 | n.a. |
| Δpks EcN | Kanamycin | Escherichia coli Nissle 1917: ΔclbA-clbQ* | n.a |
| SYNB1618 | None | Escherichia coli Nissle 1917: ΔdapA, Δprophage, ΔlacZ, lacZ::PfnrS-pheP, agaI/rsmI::PfnrS-pheP, araBC::PBAD-LAAD, malEK::PfnrS-PAL, malPT::PfnrS-PAL, yicS/nepI::PfnrS-PAL, exo/cea::lacI-Ptac-PAL, rhtBC::lacI-Ptac-PAL | PRJNA482064 |
| Δpks SYNB1618 | None | Escherichia coli Nissle 1917: ΔdapA, Δprophage, ΔlacZ, lacZ::PfnrS-pheP, agaI/rsmI::PfnrS-pheP, araBC::PBAD-LAAD, malEK::PfnrS-PAL, malPT::PfnrS-PAL, yicS/nepI::PfnrS-PAL, exo/cea::lacI-Ptac-PAL, rhtBC::lacI-Ptac-PAL, ΔclbA-clbQ | n.a |
| SYNB1934 | None | Escherichia coli Nissle 1917, ΔdapA, Δprophage, rhtB/C::lacI-Ptac-pheP, araBC::PBAD-LAAD, malK/phage tail protein::lacI-Ptac-mPAL, yicS/nepI::lacI-Ptac-mPAL, aga/rsmI::lacI-Ptac-mPAL, exo/cea::lacI-Ptac-mPAL | PRJNA749270 |
| Δpks SYNB1934 | None | Escherichia coli Nissle 1917, ΔdapA, Δprophage, rhtB/C::lacI-Ptac-pheP, araBC::PBAD-LAAD, malK/phage tail protein::lacI-Ptac-mPAL, yicS/nepI::lacI-Ptac-mPAL, aga/rsmI::lacI-Ptac-mPAL, exo/cea::lacI-Ptac-mPAL, ΔclbA-clbQ | n.a |
| SYNB8802 | None | Escherichia coli Nissle 1917, ΔthyA, Δprophage, agaI/rsmI:: PfnrS−scaaE3-oxdc-frc, exo/cea:: PfnrS-oxlT | CP087958 |
| Δpks SYNB8802 | None | Escherichia coli Nissle 1917, ΔthyA, Δprophage, agaI/rsmI:: PfnrS-scaaE3-oxdc-frc, exo/cea:: PfnrS-oxlT, ΔclbA-clbQ | n.a |
List of strains used and their relevant antibiotic resistance (where applicable).
*Δpks = ΔclbA-clbQ. Gene names separated by “/” designate the genomic regions flanking the insertion of the indicated gene.