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. Author manuscript; available in PMC: 2023 Feb 2.
Published in final edited form as: Nat Biomed Eng. 2021 Aug 2;5(10):1228–1238. doi: 10.1038/s41551-021-00770-5

ED Fig. 5. Flow cytometry of neutrophils and eosinophils.

ED Fig. 5

A, Cells were gated on scatter (FSC_A, SSC_A) followed by doublet discrimination (FSC_A, FSC_H), selection of CD45+ live cells (Fixable Yellow-, CD45+), and selection of myeloid cells (CD3-, CD11b+). This population was used to identify eosinophils (Ly6g low, Siglec F+) and neutrophils (Ly6g+, Siglec F-) of correct size (FSC_A, SSC_A). B, Numbers of CD45+ cells, eosinophils, and neutrophils from ECM, PCL, or saline treated animals one week after surgery (top). Eosinophil and neutrophil amounts as proportion of CD45+ cells are given below. Data are mean ± SEM. Statistics shown are after analysis of variance (ANOVA) followed by Dunnett’s multiple comparison testing where P is adjusted p value.