Table 1.

Photophysical properties at room temperature

Dye	Solventa	${\mathit{\lambda }}_{\mathbf{m}\mathbf{a}\mathbf{x}}^{\mathbf{a}\mathbf{b}\mathbf{s}}$ (nm)	${\mathit{\lambda }}_{\mathbf{m}\mathbf{a}\mathbf{x}}^{\mathbf{e}\mathbf{m}}$ (nm)	ε (M−1cm−1)	ΦF (%)b	Stable to thiol
ICG	PBS	780	805	64,180	2.9	Yes
	FBS	800	819	159,000	9.7
	DMSO	794	825	159,000	19.0
CW800-SO 3	PBS	774	791	190,870	10.5	No
1	PBS	805	813	19,790	1.0	No
	DMSO	818	840	160,310	15.4
2	PBS	809	827	172,980	2.3	Yes
	DMSO	817	841	214,800	11.9
3	PBS	803	823	180,540	2.3	Yes
	FBS	804	826	179,000	-
	DMSO	809	837	191,320	10.8

^aPBS = pH 7.4 1X phosphate buffered saline; FBS = fetal bovine serum; DMSO = dimethyl sulfoxide.

^bAbsolute fluorescence quantum yield measured by photon counting.