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. Author manuscript; available in PMC: 2023 Feb 2.
Published in final edited form as: Cell Rep. 2022 Jan 11;38(2):110240. doi: 10.1016/j.celrep.2021.110240

Figure 2. MEC-specific Zeb1 deficiency compromises ductal branching morphogenesis (A–C) Whole-mount analysis of MGs from 8-week-old.

Figure 2.

(A) and 17-week-old (B and C) virgin K14-Cre;Zeb1f/+ (control) and K14-Cre;Zeb1f/f (MSKO) mice. Boxed areas in (B) are shown at higher magnification.

(D and E) H&E images of MGs from 17-week-old virgin control and MSKO mice. Arrows indicate individual ductal sections.

(F and G) Whole-mount analysis of MGs from pregnant (P14.5) control and MSKO mice. Representative images are shown in (A), (B), and (F), and quantification from multiple pairs are shown in (C) (n = 3) and (G) (n = 3).

(H–K) Results of cleared fat pad transplantation of FACS-sorted basal MECs infected with lentiviruses that express shScr or shZeb1. (H) and (J) show representative images of transplants from virgin hosts analyzed at 8 weeks after transplantation (H) or hosts that were subsequently mated to WT males (J). (I) and (K) show summary of data for (H) (n = 5) and (J) (n = 2), respectively. Data presented here were obtained using shZeb1-1, and similar results were obtained using shZeb1-2 (Figures S2J–S2M).

Scale bars: 5 mm in (A), (B), and (F), 1 mm in (D), 100 μm in (E), and 5 mm in (H) and (J). See also Figure S2.